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Product Protein. Determination. USA. B.

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 06.99
PATENT ASSIGNEE This data is not available for free
PATENT CLAIMS In an assay for determn. of protein in an aq. test fluid which comprises combining fluid with molybdate salt & pyrogallol red to form a complex with molybdate ion & the absorption band of complex is shifted in the presence of protein to provide a detectable response with good resolution at protein concn. < 150 mg/dL, the IMPROVEMENT which comprises
- Introducing into the assay a substituted phenolsulfonephtha
lein dye having a pKa which enables it to operate at pH 1 - 3
& whose affinity for protein is such that it will provide a detec
table response only in the presence of > 50 mg/dL protein
to thereby render the assay suitable for detection of total
protein in test fluid
PATENT PHOTOCOPY Available on request

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