PATENT ASSIGNEE'S COUNTRY | USA |
UPDATE | 07.00 |
PATENT ASSIGNEE | This data is not available for free |
PATENT CLAIMS |
Method of amplifying single stranded first nucleic acid in a sample potentially contg said acid - Forming an immersion prodt comprising said sample & at least one primer set of nucleic acids, said set comprising at least one second & third nucleic acids comprising diffe rent seq & being covalently affixed to a support & separated on said support by a distance less than the length of said first acid, said second acid capable of forming a hybridiza tion prodt with first acid, said third acid hybridizable with acid that is complementary to first acid - Forming hybridization prodt comprising first acid & second acid, in the event said first acid is present, by imposing hybridization conditions on the immersion prodt - Forming a first amplification prodt comprising said second acid extended by nucleotides complementary to first acid, said first amplification prodt being capable of hybridiza tion with third acid - Denaturing first amplification prodt from first acid - Forming second hybridization prodt comprising said first amplification prodt & third acid by imposing hybridization conditions on immersion prodt - Forming a second amplification prodt comprising said third acid extended by nucleotides complementary to said first amplification prodt |
PATENT PHOTOCOPY | Available on request |
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