Main > CLINICAL. CHEMISTRY > Immunofluorescence Assay > Recombinant Antigen (Protein) > Insoluble Form Use

Product Canada. C

PATENT ASSIGNEE'S COUNTRY Canada
UPDATE 02.00
PATENT ASSIGNEE This data is not available for free
PATENT CLAIMS Method for detection of disease state which comprises using an insoluble form of recombinant protein in flow cytometric immunofluorescence assay, comprising
(i) Obtaining biol fluid sample within which the disease state is to be detected
(ii) Adding insoluble dorm of recombinant protein to sample to form mixt, wherein said insoluble form being produced in particle form by expression in baculovirus & wherein said insoluble form is the carrier & the antigen
(iii) Incubating mixt to permit association
(iv) Washing
(v) Adding a labelled Ab to mixt (iv) & incubating for said labeled Ab to bind with Ab within said incubated mixt to form labeled mixt
(vi) Washing
(vii) Detecting IgM complexes formed in said washed labeled mixt using flow cytometric immunofluorescence &
(viii) Correlating detection of IgM complexes with a disease state, wherein an increase level of IgM over control level is indicative of disease state
PATENT PHOTOCOPY Available on request

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