| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 06.99 |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT CLAIMS |
Method for formation of differentiated & stratified cornea & gingiva tissue comprising steps - Isolation of basal stem cells from tissue using soln.: -- N-(2-OH-Et-) piperazine-N'-(2-ethanesulfonic acid) (I) at concn. 16 - 22 mM -- NaCl (90 - 140 mM) -- Protease - Recovering isolated basal stem cells using soln -- (I) at concn 16 - 22 mM -- NaCl (90 - 140 mM) -- Protease inhibitor - Culturing said isolated basal stem cells in a medium to form a confluent sheet of undifferentiated tissue, said medium comprising -- (I) at concn 14 - 22 mM -- NaCl (100 - 120 mM) -- Histidine (0.1 - 0.25 mM) -- Isoleucine (0.05 - 0.5 mM) -- Methionine (0.1 - 0.5 mM) -- Phenylalanine (0.1 - 0.5 mM) -- Tryptophan (0.05 - 0.5 mM) -- Tyrosine (0.1 - 0.5 mM) -- Insulin-like growth factor -1 (0.3 - 30 ng/ml) - Culturing said sheet of undifferentiated tissue in a differentia tion medium to form a sheet of undifferentiated & stratified tissue using a medium comprising -- (I) at concn 14 - 22 mM -- NaCl (100 - 120 mM) -- Ca(II) ions (0.7 - 3 mM) -- Histidine (0.1 - 0.25 mM) -- Isoleucine (0.05 - 0.5 mM) -- Methionine (0.1 - 0.5 mM) -- Phenylalanine (0.1 - 0.5 mM) - Tryptophan (0.05 - 0.5 mM) -- Tyrosine (0.1 - 0.5 mM) -- Beta transforming growth factor (3 - 30 ng/ml) |
| PATENT PHOTOCOPY | Available on request |
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