| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 07.99 |
| PATENT ASSIGNEE | Scripps Research Institute |
| PATENT CLAIMS |
Process for using PAPS in an enzyme catalyzed sulfation of an acceptor with recycling of phosphorylated adenosine intermediates comprising admixing following ingredients in aq medium contg Mg & K ions within single vessel to form aq reaction medium - 3'-Nucleotidase - ATP sulfurylase - APS kinase - Pyrophosphorylase - PAPS - Sulfate ion - ATP-regenerating system comprising phosphate donor & phosphorylating enzyme - Sulfate acceptor for said sulfotransferase The concn of sulfate ion > than concn of PAPS & activity of 3' nucletidase being < than that of (ii)-(v) & - Maintaining aq reaction medium at pH 5-10 at 0-40C |
| PATENT PHOTOCOPY | Available on request |
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